Experimental handling and colonic temperature measurement have been shown to cause stress and induce a long-lasting rise in colonic temperature in the rat. This stress-induced hyperthermia was blocked by microinjection of the narcotic antagonist naltrexone into the preoptic-anterior hypothalamus (POAH) of the brain, but was not significantly affected by similar injections into areas of the brain above the POAH. Thus, the stress-induced hyperthermia may be caused by activation of the endogenous opioid mechanism in the POAH.