Laser therapy's interaction with living organisms, especially in the infrared range, is crucial for medical applications. This study proposed the effects of an 810 nm high-power laser on several parameters of human blood. Blood samples from ten individuals (five females and five males) were analyzed in three stages: baseline, laser irradiation, and irradiation after a 30-day intake of 300 mg vitamin C and 10 mg zinc. Data normality using the Shapiro-Wilk test, homogeneity of variances was evaluated with Levene's test, followed by parametric tests such as t-tests for analysis. This study examines the effects of a 1 W infrared continuous wave laser on blood parameters, focusing on its damage threshold. Blood samples, covered by chicken skin, were irradiated at distances of 1 cm and 6 cm from the laser emission source, using spot sizes of 3 and 15 cm² with an 810 nm diode laser positioned perpendicularly to the stent base. The calculated power density was 500 W/cm² at 1 cm and 7.69 W/cm² at 6 cm. The total energy delivered at both distances during 30 and 120 s was 15 J and 60 J, respectively. White blood cell (WBC) counts were measured using automated hematology analyzers, revealing the highest counts on the initial day and the lowest on day 30. The red blood cell (RBC) index also showed significant elevation on the first day, with no notable change by day 30. 810 nm laser therapy significantly impacts blood parameters, including WBC and RBC indices.
Keywords: Blood cells; Cell survival; Experimental design; Infrared laser radiation; Optical parameters.
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