MAPK signaling mediates tamoxifen resistance in estrogen receptor-positive breast cancer

Mol Cell Biochem. 2025 Sep;480(9):4973-4989. doi: 10.1007/s11010-025-05304-0. Epub 2025 May 23.

Abstract

Tamoxifen is a cornerstone in the treatment of estrogen receptor (ER)-positive breast cancer, yet resistance to this therapy remains a significant clinical challenge. In most cases, the resistance phenotype is not caused by loss or mutation of the ER, but by changes in multiple proliferative and survival pathways. The mitogen-activated protein kinase (MAPK) signaling pathways regulate various cellular processes such as cell growth, proliferation, and apoptosis. This review provides a comprehensive analysis of molecular mechanisms that sustain MAPK activation and promote tamoxifen resistance. We evaluated molecular factors that promote the survival of tamoxifen-resistant cells through the regulation of MAPK signaling, including growth factors, RNA-binding proteins, non-genomic ER variants, and microRNAs. Mitochondrial dynamics and their regulation by MAPK highlight novel adaptive mechanisms employed by resistant cells to survive. Furthermore, MAPK-mediated phosphorylation of ERα enhances resistance through ligand-independent activation and sustained cellular proliferation. MAPK and parallel oncogenic pathways, including PI3K/AKT and receptor tyrosine kinases (EGFR, IGF-1R, and FGFR), function synergistically to enhance signaling redundancy and compensatory survival mechanisms. Therapeutic interventions targeting MAPK signaling-ranging from small-molecule inhibitors to RNA-based therapies-offer promising avenues for overcoming tamoxifen resistance.

Keywords: Drug resistance; Estrogen; MAPK; Tamoxifen resistance.

Publication types

  • Review

MeSH terms

  • Antineoplastic Agents, Hormonal / therapeutic use
  • Breast Neoplasms* / drug therapy
  • Breast Neoplasms* / metabolism
  • Breast Neoplasms* / pathology
  • Drug Resistance, Neoplasm* / drug effects
  • Female
  • Humans
  • MAP Kinase Signaling System* / drug effects
  • Neoplasm Proteins* / metabolism
  • Receptors, Estrogen* / metabolism
  • Tamoxifen* / pharmacology
  • Tamoxifen* / therapeutic use

Substances

  • Tamoxifen
  • Receptors, Estrogen
  • Neoplasm Proteins
  • Antineoplastic Agents, Hormonal