Discovery of a gamma-carboxyglutamic acid-containing protein in human spermatozoa

FEBS Lett. 1985 Oct 7;190(1):137-41. doi: 10.1016/0014-5793(85)80444-0.

Abstract

Here we describe the identification of a gamma-carboxyglutamic acid-containing protein in human spermatozoa. After thermal decarboxylation the protein is a good substrate for vitamin K-dependent carboxylase from various origins. A quick purification procedure for the decarboxylated protein is presented and in a preliminary characterization we have established its Mr (28 000-30 000) and its amino acid composition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Carboxyglutamic Acid / analysis*
  • Amino Acids / analysis
  • Chemical Phenomena
  • Chemistry
  • Chromatography, High Pressure Liquid
  • Decarboxylation
  • Glutamates / analysis*
  • Humans
  • Male
  • Proteins / isolation & purification*
  • Spermatozoa / analysis*

Substances

  • Amino Acids
  • Glutamates
  • Proteins
  • 1-Carboxyglutamic Acid