Polyadenylation of a human mitochondrial ribosomal RNA transcript detected by molecular cloning

Gene. 1985;35(3):305-12. doi: 10.1016/0378-1119(85)90009-5.


We have identified by molecular cloning a polyadenylated RNA transcript in the human leukemia cell line, K562, which is complementary to a portion of the gene-encoding mitochondrial 16S ribosomal RNA (mt 16S rRNA). The cloned portion of the transcript corresponds to positions 2191-2395 of the human mt genome. The clone represents a cDNA copy of an RNA transcript from the H strand and carries an additional poly(A) tail 21 residues long at its 3'-end. Our data provide direct evidence for polyadenylation of some mt 16S rRNA transcripts.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cell Line
  • Humans
  • Leukemia
  • Mitochondria / physiology*
  • Poly A / genetics*
  • RNA Processing, Post-Transcriptional
  • RNA, Ribosomal / genetics*


  • RNA, Ribosomal
  • Poly A