Isolation and Culture of Osteoblasts

Lucie E Bourne; Isabel R Orriss

doi:10.1007/978-1-0716-4306-8_1

Isolation and Culture of Osteoblasts

Methods Mol Biol. 2025:2885:3-22. doi: 10.1007/978-1-0716-4306-8_1.

Authors

Lucie E Bourne¹, Isabel R Orriss²

Affiliations

¹ Centre for Lifelong Health, School of Applied Sciences, University of Brighton, Brighton, UK.
² Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK. iorriss@rvc.ac.uk.

PMID: 40448753
DOI: 10.1007/978-1-0716-4306-8_1

Abstract

This chapter describes the isolation, culture, and staining of primary osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. While the focus is on rodent and human osteoblasts, this chapter will also cover the culture of osteoblasts from less conventional animal species.

Keywords: Bone formation; Mineralization; Osteoblast.

MeSH terms

Animals
Cell Culture Techniques* / methods
Cell Differentiation
Cell Separation* / methods
Cells, Cultured
Humans
Mice
Osteoblasts* / cytology
Osteoblasts* / metabolism
Rats