Zinc-mediated metalloimmunotherapy with dual elimination of tumor and intratumoral bacteria in oral squamous cell carcinoma

Abstract

Metal dyshomeostasis has been implicated in the immune evasion, host-microbiota interaction and tumor progression during carcinogenesis. Specifically, zinc dyshomeostasis has been observed in oral squamous cell carcinoma (OSCC), and strongly associated with Porphyromonas gingivalis (P. gingivalis) infection. Therefore, developing a zinc-mediated metalloimmunotherapy with synergistic antitumor, antibacteria and immunoactivation effects could be an efficient strategy to combat OSCC. Herein, PYT@ZIF8@siRNA nanoparticles (NPs) were constructed from zeolite imidazolate framework-8 (ZIF8) NPs loaded with pyrithione (PYT, a zinc ionophore) and small interfering RNA (siRNA) for SLC30A1 (zinc transporter 1). Zinc overload caused by PYT@ZIF8@siRNA NPs effectively eradicated OSCC cells by causing mitochondrial dysfunction and pro-death mitophagy, and simultaneously eliminated P. gingivalis by destroying the membrane structure and elevating the reactive oxygen species. Concurrently, PYT@ZIF8@siRNA NPs evoked robust antitumor immunity by eliciting immunogenic cell death and attenuating immunosuppression mediated by P. gingivalis. Moreover, PYT@ZIF8@siRNA significantly interrupted epithelial malignant transformation in experimental oral carcinogenesis model, and synergized with programmed death-1 blockade to establish a collaborative tumoricidal network by reprogramming the immunosuppressive tumor microenvironment in P. gingivalis-infected tumor model. In all, our work proposes a strategy for the simultaneous elimination of tumor and intratumoral P. gingivalis, providing a new avenue for precision metalloimmunotherapy in OSCC.

Keywords: Immunogenic cell death; Metal-organic framework; Metalloimmunotherapy; Oral squamous cell carcinoma; Porphyromonas gingivalis; Tumor microenvironment; Zinc homeostasis.