Mutagen-directed attenuation of Rift Valley fever virus as a method for vaccine development

J Gen Virol. 1985 Oct;66 ( Pt 10):2271-7. doi: 10.1099/0022-1317-66-10-2271.

Abstract

Serial mutagenesis with 5-fluorouracil has been employed to derive an attenuated strain of Rift Valley fever virus for use as a live virus vaccine.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bunyaviridae / immunology*
  • Cell Line
  • Fluorouracil / pharmacology
  • Humans
  • Lethal Dose 50
  • Mutation*
  • Rift Valley fever virus / genetics
  • Rift Valley fever virus / growth & development
  • Rift Valley fever virus / immunology*
  • Rift Valley fever virus / pathogenicity
  • Vaccines, Attenuated
  • Viral Plaque Assay
  • Viral Vaccines*
  • Virulence

Substances

  • Vaccines, Attenuated
  • Viral Vaccines
  • Fluorouracil