Mucins and Bacterial Interactions

Yannick Rossez; Renaud Léonard; Olivier Vidal; Zina Chouit; Yann Guerardel; Edwige Madec; Catherine Robbe-Masselot

doi:10.1007/978-1-0716-4627-4_15

Mucins and Bacterial Interactions

Methods Mol Biol. 2025:2942:177-186. doi: 10.1007/978-1-0716-4627-4_15.

Authors

Yannick Rossez¹, Renaud Léonard², Olivier Vidal², Zina Chouit², Yann Guerardel², Edwige Madec², Catherine Robbe-Masselot²

Affiliations

¹ Faculté des sciences et technologies, UMR 8576 CNRS, Unité de glycobiologie structurale et fonctionnelle, Villeneuve d'Ascq, France. yannick.rossez@univ-lille.fr.
² Faculté des sciences et technologies, UMR 8576 CNRS, Unité de glycobiologie structurale et fonctionnelle, Villeneuve d'Ascq, France.

PMID: 40498316
DOI: 10.1007/978-1-0716-4627-4_15

Abstract

The handling of high-molecular-weight mucins requires specialized methods. The present manuscript introduces a slot blotting-based technique to screen post-ultracentrifugation fractions using colorimetric assays. Next, we describe an easy slot blot-based method to characterize mucin-bacteria interactions and to assess the effects of glycan enzymatic treatment on bacterial adhesion.

Keywords: Adhesion; Bacteria; Glycans; Mucin; Slot blot.

MeSH terms

Bacteria* / metabolism
Bacterial Adhesion*
Colorimetry / methods
Humans
Mucins* / chemistry
Mucins* / metabolism
Polysaccharides / metabolism

Substances

Mucins
Polysaccharides