Monoclonal antibodies against human liver cytochrome P-450

Biochem Pharmacol. 1985 Oct 1;34(19):3547-52. doi: 10.1016/0006-2952(85)90732-4.


Monoclonal hybridomas which produce antibodies against human liver microsomal cytochrome P-450 were developed. Three similar hybridomas produced antibodies which recognized an epitope specific to a family of human P-450 isozymes (P-450(5)). This epitope was also present on cytochrome P-450 PCN-E (pregnenolone-16 alpha-carbonitrile induced) from rat liver microsomes, but this isozyme differed from the human P-450(5) by its molecular weight. These antibodies enabled us to quantify cytochrome P-450(5) in human liver microsomes and to demonstrate an important quantitative polymorphism in the human liver monooxygenase system.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Cytochrome P-450 Enzyme System / analysis
  • Cytochrome P-450 Enzyme System / immunology*
  • Female
  • Humans
  • Hybridomas / immunology
  • Isoenzymes / immunology*
  • Liver / embryology
  • Mice
  • Mice, Inbred BALB C
  • Microsomes, Liver / enzymology*
  • Rats
  • Species Specificity


  • Antibodies
  • Antibodies, Monoclonal
  • Isoenzymes
  • Cytochrome P-450 Enzyme System