Folic Acid Targeted Selenium-Curcumin Nanoparticles to Enhance Apoptosis in Breast Cancer Cells

Chem Biodivers. 2025 Oct;22(10):e00183. doi: 10.1002/cbdv.202500183. Epub 2025 Jun 16.

Abstract

Despite advancements in drug delivery, breast cancer remains a leading cause of mortality, and creating therapeutic systems that balance efficacy and safety remains a challenge. Selenium (Se) and curcumin (Cur) exhibit antioxidant and anticancer properties, but their efficient application in cancer therapy is complex. This study developed a targeted drug delivery system for 4T1 breast cancer cells using selenium nanoparticles (SeNPs) loaded with curcumin and coated with chitosan-folic acid (Cs-FA) to enhance targeting efficiency. The incorporation of Cs-FA and Cur increased nanoparticle size from 105 to 184.2 nm and reduced 4T1 cell viability by 40% at a concentration of 40 µg/mL. The Cs-FA coating significantly enhanced apoptosis, as evidenced by upregulated p53 and downregulated BCL2 gene expression compared to other formulations. These results suggest that Se@Cur/Cs-FA nanoparticles effectively induce apoptosis, highlighting their potential as a promising cancer therapy.

Keywords: apoptosis; cancer therapy; drug delivery; natural products; selenium nanoparticles.

MeSH terms

  • Animals
  • Antineoplastic Agents* / chemistry
  • Antineoplastic Agents* / pharmacology
  • Apoptosis* / drug effects
  • Breast Neoplasms* / drug therapy
  • Breast Neoplasms* / metabolism
  • Breast Neoplasms* / pathology
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chitosan / chemistry
  • Curcumin* / chemistry
  • Curcumin* / pharmacology
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Female
  • Folic Acid* / chemistry
  • Folic Acid* / pharmacology
  • Humans
  • Mice
  • Nanoparticles* / chemistry
  • Particle Size
  • Selenium* / chemistry
  • Selenium* / pharmacology

Substances

  • Curcumin
  • Selenium
  • Folic Acid
  • Antineoplastic Agents
  • Chitosan