Double synchronization of human lymphocyte cultures: selection for high-resolution banded metaphases in the first and second division

Cytogenet Cell Genet. 1985;39(4):292-5. doi: 10.1159/000132160.


The present report describes a double synchronization technique that selects for first- and second-division metaphases in human lymphocyte cultures. The technique ensures high mitotic indices without elaborate handling of cultures. Combined with appropriate band induction, this technique provides a high yield of uniform metaphases with well-defined high-resolution banding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Chromosome Banding
  • Humans
  • Karyotyping
  • Lymphocytes / cytology
  • Lymphocytes / ultrastructure*
  • Metaphase*