Psychometric functions for the recognition of vernier displacements have been measured in 6 strabismic amblyopes, 1 anisometropic amblyope, and 1 bilateral amblyope of unknown aetiology. In the non-amblyopic eyes of the 7 unilateral amblyopes the mean threshold vernier offset was about half of that of a group of 7 experienced normal observers. No correlation was found between vernier acuities and Snellen acuities in the abnormal eyes of squinters. These eyes also displayed massive distortions of horizontal spatial values causing perceptual distortions. In all but one of the amblyopic eyes tested hyperacuity virtually disappeared at the brief exposure duration of 50 ms. None of the observed effects could be predicted from the amblyopic contrast sensitivities, grating resolutions, or letter acuities. This suggests that the loss of positional information is an independent and most significant feature of amblyopic visual dysfunction.