The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria

J Biol Chem. 1985 Oct 25;260(24):12998-3002.

Abstract

The flavoenzymes dimethylglycine dehydrogenase (EC 1.5.99.2) and sarcosine dehydrogenase (EC 1.5.99.1) contain covalently bound FAD linked via the 8 alpha-position of the isoalloxazine ring to the imidazole N(3) of a histidine residue (Cook, R. J., Misono, K. S., and Wagner, C. (1984) J. Biol. Chem. 259, 12475-12480). The flavin-peptides from tryptic digests of these two enzymes have been isolated and sequenced. Automated sequence analysis showed that the flavin-peptide from dimethylglycine dehydrogenase contained 25 amino acid residues in the following sequence: Ser-Glu-Leu-Thr-Ala-Gly-Ser- Thr-Trp-His(flavin)-Ala-Ala-Gly-Leu-Thr-Thr-Tyr-Phe-His-Pro-Gly-Ile-A sn-Leu-Lys. The sequence determined for the flavin-peptide from sarcosine dehydrogenase contained 14 amino acid residues Leu-Thr-Ser-Gly-Thr-Thr-Trp-His(flavin)-Thr-Ala-Gly-Leu-Gly-Arg.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Dimethylglycine Dehydrogenase
  • Flavin-Adenine Dinucleotide*
  • Male
  • Mitochondria, Liver / enzymology*
  • Mitochondrial Proteins
  • Oxidoreductases, N-Demethylating* / metabolism
  • Peptide Fragments* / metabolism
  • Rats
  • Rats, Inbred Strains
  • Sarcosine Dehydrogenase
  • Spectrophotometry
  • Trypsin / metabolism

Substances

  • Mitochondrial Proteins
  • Peptide Fragments
  • Flavin-Adenine Dinucleotide
  • Oxidoreductases, N-Demethylating
  • Sarcosine Dehydrogenase
  • Sardh protein, rat
  • Dimethylglycine Dehydrogenase
  • Dmgdh protein, rat
  • Trypsin