Revealing hyperactivated IFN-γ pathways in perianal fistulizing Crohn's disease using single-cell and spatial multi-omics

J Clin Invest. 2025 Jul 3;135(17):e193413. doi: 10.1172/JCI193413. eCollection 2025 Sep 2.

Abstract

Up to 40% patients with Crohn's disease suffer from perianal disease, a debilitating complication with unclear etiology. This study identified unique pathophysiology which may help develop novel therapies.

Keywords: Cellular immune response; Gastroenterology; Immunology; Inflammatory bowel disease; Therapeutics.

MeSH terms

  • Adult
  • Crohn Disease* / complications
  • Crohn Disease* / genetics
  • Crohn Disease* / metabolism
  • Crohn Disease* / pathology
  • Female
  • Humans
  • Interferon-gamma* / genetics
  • Interferon-gamma* / metabolism
  • Male
  • Multiomics
  • Rectal Fistula* / etiology
  • Rectal Fistula* / genetics
  • Rectal Fistula* / metabolism
  • Rectal Fistula* / pathology
  • Signal Transduction*
  • Single-Cell Analysis*

Substances

  • Interferon-gamma
  • IFNG protein, human