Acute colitis produced by chemotactic peptides in rats and mice

Am J Pathol. 1985 Nov;121(2):284-90.


Colonic inflammation was produced in rats and mice by peptides chemotactic for polymorphonuclear leukocytes. Instillation of formylmethionyl-leucyl-phenylalanine (FMLP) and formylnorleucyl-leucyl-phenylalanine (FNLP) into isolated segments of rat colon caused marked mucosal edema and polymorphonuclear leukocyte infiltration within 2 hours. Higher concentrations of FNLP caused ulceration and necrosis as well. Formylmethionine (FMet), a compound with less chemotactic activity, caused much less inflammation. In mice, rectal instillation of FNLP caused dose-dependent acute mucosal inflammation which persisted for longer than 12 hours. Twice-weekly rectal instillation of FNLP provided a model of colitis based on neutrophil chemotaxis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Chemotaxis, Leukocyte
  • Colitis / etiology*
  • Colitis / immunology
  • Colitis / pathology
  • Male
  • Mice
  • Mice, Inbred Strains
  • N-Formylmethionine Leucyl-Phenylalanine*
  • Neutrophils / immunology
  • Rats
  • Rats, Inbred Strains


  • N-Formylmethionine Leucyl-Phenylalanine