Single GNAS Droplet-Based Digital Polymerase Chain Reaction Analysis of Pancreatic Cyst Fluid: An Effective Up-Front Strategy for Mucinous Cyst Diagnosis by Endoscopic Ultrasound-Guided Fine-Needle Aspiration

Clin Transl Gastroenterol. 2025 Sep 1;16(9):e00887. doi: 10.14309/ctg.0000000000000887.

Abstract

Introduction: Accurate diagnosis of mucinous pancreatic cystic neoplasms (mPCNs) remains a clinical challenge. This study investigated the utility of single GNAS droplet-based digital polymerase chain reaction (ddPCR) analysis as a novel approach to refine the diagnostic accuracy of mPCNs using endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA).

Methods: Patients who underwent EUS-FNA and GNAS pancreatic cyst fluid (PCF) analyses for pancreatic cystic lesion (PCL) assessment were prospectively enrolled. Cysts were categorized as mPCNs, non-mPCNs, or inconclusive PCLs (iPCLs) by integrating increasing information levels: high-resolution imaging and non-DNA PCF features (level 1), GNAS PCF analysis (level 2), and surgical pathology (level 3).

Results: One hundred forty patients were included, 25 of whom underwent pancreatic surgery. Level 1 identified 68 mPCNs (49%), 24 non-mPCNs (17%), and 48 iPCLs (34%). GNAS mutations were detected in 42 of 68 (62%) mPCNs, 1 of 24 (4%) non-mPCNs, and 16 of 48 (33%) iPCLs. Level 2 increased mPCN detection to 62% and reduced iPCLs by one-third. Mutated GNAS showed 66% sensitivity for diagnosing mPCNs in the whole cohort and 65% in resected cases, outperforming both imaging and non-DNA PCF mucinous criteria, with 100% specificity and limited concordance with carcinoembryonic antigen, cytology, and fluid viscosity, highlighting its complementary diagnostic value. Cost-effectiveness simulations for iPCLs demonstrated that GNAS -ddPCR significantly reduced diagnostic costs by 24% compared with next-generation sequencing testing.

Discussion: Single GNAS- ddPCR analysis in PCF supported mPCNs diagnosis in 62% of cases and uncovered 33% of iPCLs as mPCNs with 100% specificity. It adds complementary value to standard cyst fluid markers offering a simple and cost-effective tool for improving PCL diagnosis by EUS-FNA.

Keywords: ddPCR; droplet-based digital PCR; molecular analysis; pancreatic cystic lesions.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / genetics
  • Chromogranins* / analysis
  • Chromogranins* / genetics
  • Cyst Fluid / chemistry
  • Endoscopic Ultrasound-Guided Fine Needle Aspiration
  • Female
  • GTP-Binding Protein alpha Subunits, Gs* / analysis
  • GTP-Binding Protein alpha Subunits, Gs* / genetics
  • Humans
  • Male
  • Middle Aged
  • Pancreas / diagnostic imaging
  • Pancreas / pathology
  • Pancreas / surgery
  • Pancreatic Cyst* / diagnosis
  • Pancreatic Cyst* / pathology
  • Pancreatic Neoplasms* / diagnosis
  • Pancreatic Neoplasms* / genetics
  • Pancreatic Neoplasms* / pathology
  • Pancreatic Neoplasms* / surgery
  • Polymerase Chain Reaction* / methods
  • Prospective Studies
  • Sensitivity and Specificity

Substances

  • GNAS protein, human
  • GTP-Binding Protein alpha Subunits, Gs
  • Chromogranins
  • Biomarkers, Tumor