A Mass Spectrometry-Based Pipeline for Analyzing RNA Modifications in Cells and Tissues

Aurore Attina; Jérôme Vialaret; Christophe Hirtz; Alexandre David

doi:10.1007/978-1-0716-4726-4_14

A Mass Spectrometry-Based Pipeline for Analyzing RNA Modifications in Cells and Tissues

Methods Mol Biol. 2025:2962:199-213. doi: 10.1007/978-1-0716-4726-4_14.

Authors

Aurore Attina¹, Jérôme Vialaret¹, Christophe Hirtz¹, Alexandre David^{2

3}

Affiliations

¹ IRMB-PPC, INM, CHU Montpellier, INSERM, Université de Montpellier, CNRS, Montpellier, Occitanie, France.
² IRMB-PPC, INM, CHU Montpellier, INSERM, Université de Montpellier, CNRS, Montpellier, Occitanie, France. alexandre.david@inserm.fr.
³ IRCM, Université de Montpellier, ICM, Inserm, Montpellier, Occitanie, France. alexandre.david@inserm.fr.

PMID: 40699431
DOI: 10.1007/978-1-0716-4726-4_14

Abstract

Mass spectrometry has become indispensable in studying post-transcriptional modifications of RNA, a.k.a. epitranscriptomics, which are crucial for regulating RNA metabolism, gene expression, and major biological processes. Over 150 chemical modifications have been identified across all RNA subtypes. Dysregulation of RNA mark deposition can lead to disease development and progression. Therefore, accurately detecting and characterizing these modifications are vital for understanding their functional impact and uncovering potential therapeutic and diagnostic biomarkers. In this chapter, we describe a mass spectrometry-based method for detecting and quantifying nearly 40 different RNA modifications.

Keywords: Biomarker discovery; Multiple reaction monitoring (MRM); RNA modifications; Targeted epitranscriptomics.

MeSH terms

Humans
Mass Spectrometry* / methods
RNA Processing, Post-Transcriptional*
RNA* / chemistry
RNA* / genetics
RNA* / metabolism

Substances

RNA