Respiratory syncytial virus envelope glycoprotein (G) has a novel structure

Nucleic Acids Res. 1985 Nov 11;13(21):7795-812. doi: 10.1093/nar/13.21.7795.


Amino acid sequence of human respiratory syncytial virus envelope glycoprotein (G) was deduced from the DNA sequence of a recombinant plasmid and confirmed by limited amino acid microsequencing of purified 90K G protein. The calculated molecular mass of the protein encoded by the only long open reading frame of 298 amino acids was 32,588 daltons and was somewhat smaller than the 36K polypeptide translated in vitro from mRNA selected by this plasmid. Inspection of the sequence revealed a single hydrophobic domain of 23 amino acids capable of membrane insertion at 41 residues from the N-terminus. There was no N-terminal signal sequence and the hydrophilic N-terminal 20 residues probably represent the cytoplasmic tail of the protein. The N-terminally oriented membrane insertion was somewhat analogous to paramyxovirus hemagglutinin-neuraminidase (HN) and influenza neuraminidase (NA). The protein was moderately hydrophilic and rich in hydroxy-amino acids. It was both N- and O-glycosylated with the latter contributing significantly to the net molecular mass 90K.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carcinoma, Squamous Cell
  • Cell Line
  • Humans
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Plasmids
  • Protein Biosynthesis
  • RNA, Messenger / genetics
  • Respiratory Syncytial Viruses / genetics*
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / isolation & purification


  • RNA, Messenger
  • Viral Envelope Proteins

Associated data

  • GENBANK/K01459
  • GENBANK/K02719
  • GENBANK/K03348
  • GENBANK/K03349
  • GENBANK/M11217
  • GENBANK/M11244
  • GENBANK/M11486
  • GENBANK/M11487
  • GENBANK/M11505
  • GENBANK/M11514
  • GENBANK/M11631
  • GENBANK/M12966
  • GENBANK/X00001
  • GENBANK/X02221