Twenty male cats were treated once or twice with 1 watt/sq cm of ultrasound for 10 minutes. Each of 24 male dogs received one to three treatments with 1 watt/sq cm for 10 minutes. Another six dogs were treated with 2 watts/sq cm for 15 minutes. Four Cebus apella monkeys were treated with the same dosage as that used for the cats and dogs. A dosage of 1 watt/sq cm for 10 minutes was also applied to four human patients without the use of anesthetics, and no pain or side effects were noted. In all treated animals as well as in human patients the results indicate that ultrasound significantly suppresses spermatogenesis according to the dosage and frequency of treatment, without any effect on Leydig cells or blood testosterone levels.
PIP: Ultrasound was tested as a method of sterilization in cats, dogs, monkeys, and humans, utilizing an ultrasound machine manufactured by Whitewater Electronics specifically for this research. 30 male cats were divided into 3 groups, 2 experimental and 1 control. The treated groups received 1 watt/sq cm for 10 minutes once, or a 2nd time after 48 hours. Testicular biopsies were made 60 days after treatment. 24 male dogs were divided into 3 groups receiving either 1,2, or 3 treatments within a 1-week interval with 1 watt/sq cm for 10 minutes. Sperm counts were made for 5 consecutive weeks preceding ultrasound treatment and semen analyses made weekly. 6 dogs were treated with 2 watts/sq cm for 15 minutes. Antibodies to spermatozoa were determined by the Kibrick method. 4 South African monkeys were treated with 1 watt/sq cm for 10 minutes; testicular biopsies were obtained 1 month before treatment and 60 days after treatment. 1 watt/sq cm for 10 minutes. Testicular biopsies were obtained 3 weeks before treatment and orchiectomies were performed 14 -17 days after treatment. In all treated animals and human patients, ultrasound significantly suppresses spermatogenesis accoridng to the dosage and frequency of treatment, without any effect on Leydig cells or blood testosterone levels.