Chloroplast biogenesis: quantitative determination of monovinyl and divinyl Mg-protoporphyrins and protochlorophyll(ides) by spectrofluorometry

Anal Biochem. 1985 Aug 15;149(1):43-61. doi: 10.1016/0003-2697(85)90475-0.


General equations which permit the determination of the amounts of any two closely related fluorescent compounds which can be distinguished by 77 degrees K but not by 293 degrees K spectrofluorometry have been described. This was achieved in the presence or absence of a third interfering compound, without prior separation of the fluorescent species. The adaptation of the generalized equations to the determination of the amounts of monovinyl (MV) and divinyl (DV) Mg-protoporphyrins or of MV and DV protochlorophyll(ides) in the presence or absence of Mg-Protos [Mg-protoporphyrin IX (Mg-Proto), Mg-Proto monoester, Mg-Proto diester or a mixture of those three tetrapyrroles] interference, was then demonstrated over a wide range of MV/DV tetrapyrrole proportions. These equations are likely to be very useful for the study of the intermediary metabolism of the monovinyl and divinyl chlorophyll biosynthetic routes in plants.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chlorophyll / analogs & derivatives*
  • Chloroplasts / metabolism*
  • Mathematics
  • Photosynthesis
  • Plants / analysis
  • Porphyrins / analysis*
  • Protochlorophyllide / analogs & derivatives*
  • Protochlorophyllide / analysis*
  • Protoporphyrins / analysis*
  • Pyrroles / analysis
  • Spectrometry, Fluorescence
  • Temperature
  • Tetrapyrroles


  • Porphyrins
  • Protoporphyrins
  • Pyrroles
  • Tetrapyrroles
  • Chlorophyll
  • magnesium protoporphyrin
  • 2,4-divinylprotochlorophyllide
  • Protochlorophyllide
  • magnesium monovinylprotoporphyrin