Tunable and robust optical and structural properties of a cooperative squaraine-dye aggregate-DNA DX-DAE tile system

Nanoscale. 2025 Aug 15;17(32):18646-18677. doi: 10.1039/d5nr00863h.

Abstract

Molecular excitons, which are excitations delocalized over multiple dyes in a wavelike manner, are of interest for a wide range of applications, including quantum information science. Numerous studies have templated a variety of synthetic dyes via a DNA scaffold to induce dye aggregation to create molecular excitons upon photoexcitation. Dye aggregate optical properties are critically dependent on relative dye geometry and local environment; therefore, an understanding of dye-dye and DNA-dye interactions is critical for advancing toward more complex DNA-dye systems. The extensively studied DNA Holliday junction (HJ) and less-studied double-crossover (DX) tile motif are fundamental test beds for designing complex and ultimately modular DNA-dye architectures. Here, we report the first study of single-linked squaraine dye aggregation and exciton delocalization on a larger and more stable (compared with the HJ) DX tile motif. We first highlight a few DNA-dye constructs that support single dyes and aggregates with distinct optical properties that are both tunable-through sample design, buffer conditions, and heat treatment-and robust to environment changes, including transfer to solid phase. Next, we assess several experimental and design considerations that demonstrate directed dye-driven assembly of a novel double-tile DNA configuration. Our results demonstrate that single-linked squaraine dyes templated to DX tiles provide a viable research path to design and evaluate dye aggregate networks that support exciton delocalization. We include herein the first report of exciton delocalization in the solid phase in a DNA-dye construct. Additionally, our findings indicate that dye aggregation impacts the assembly of the DNA-dye construct, and, in some cases, thereby cooperates with the DNA to determine a final robust system configuration. Finally, we show that a controlled annealing schedule can be employed to promote the homogeneous assembly of DNA-dye constructs. The findings in this study contribute to the understanding of DNA-dye systems and the relevant factors involved in their directed assembly to achieve specific constructs with desirable properties.

MeSH terms

  • Coloring Agents* / chemistry
  • Cyclobutanes* / chemistry
  • DNA* / chemistry
  • DNA, Cruciform* / chemistry
  • Fluorescent Dyes* / chemistry
  • Nucleic Acid Conformation
  • Phenols* / chemistry

Substances

  • squaraine
  • Cyclobutanes
  • Phenols
  • DNA
  • Fluorescent Dyes
  • DNA, Cruciform
  • Coloring Agents