Advances in human myeloid-engrafting NSG-SGM3 and MISTRG mice

Hannah Stocks; Jolien Van Cleemput; Linos Vandekerckhove; Andy Wullaert

doi:10.1016/j.it.2025.07.011

Advances in human myeloid-engrafting NSG-SGM3 and MISTRG mice

Trends Immunol. 2025 Oct;46(10):662-665. doi: 10.1016/j.it.2025.07.011. Epub 2025 Aug 8.

Authors

Hannah Stocks¹, Jolien Van Cleemput², Linos Vandekerckhove³, Andy Wullaert⁴

Affiliations

¹ Cell Death Signaling Laboratory, Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium; VIB-UGent Center for Inflammation Research, VIB, 9052 Ghent, Belgium. Electronic address: hannah.stocks@uantwerpen.be.
² Laboratory of Virology, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, 9000 Ghent, Belgium; HIV Cure Research Center (HCRC), 9000 Ghent, Belgium.
³ HIV Cure Research Center (HCRC), 9000 Ghent, Belgium; Department of Internal Medicine and Paediatrics, Ghent University, 9052 Ghent, Belgium.
⁴ Cell Death Signaling Laboratory, Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium; VIB-UGent Center for Inflammation Research, VIB, 9052 Ghent, Belgium; Department of Internal Medicine and Paediatrics, Ghent University, 9052 Ghent, Belgium. Electronic address: andy.wullaert@uantwerpen.be.

PMID: 40781011
DOI: 10.1016/j.it.2025.07.011

Abstract

Humanized mouse models with transgenic expression of human myelopoiesis-supporting growth factors have enhanced human myeloid cell engraftment and improved the study of human innate immune responses. Here, we discuss the remaining challenges associated with studying innate immunity in humanized NSG-SGM3 and MISTRG mice, as well as potential advances to overcome them.

Keywords: humanized mice; immune engraftment; infection; innate immunity.

Publication types

Review

MeSH terms

Animals
Humans
Immunity, Innate
Mice
Mice, Transgenic
Myeloid Cells* / immunology
Myeloid Cells* / transplantation
Myelopoiesis* / genetics