We report the complete genome of Burkholderia pseudomallei strain 490f, isolated from a ceftazidime treatment failure case in Northeast Thailand. Oxford Nanopore and Illumina sequencing techniques revealed two circular chromosomes. A 150 kb region on the small chromosome showed high coverage and complex repeats, which were manually resolved.
Keywords: Burkholderia pseudomallei; ceftazidime resistance; melioidosis.