Proteomic Analysis of BMAC from the Iliac Crest and Humeral Head Using Support Vector Machines

Colin Herna; Madison Cipriani; Yu Zhang; Ranjan Sachdev; Sabrina Jedlicka

doi:10.1021/acsomega.5c03945

Proteomic Analysis of BMAC from the Iliac Crest and Humeral Head Using Support Vector Machines

ACS Omega. 2025 Jul 29;10(31):34975-34985. doi: 10.1021/acsomega.5c03945. eCollection 2025 Aug 12.

Authors

Colin Herna¹, Madison Cipriani¹, Yu Zhang¹, Ranjan Sachdev², Sabrina Jedlicka¹

Affiliations

¹ Lehigh University. Bioengineering, 5 E. Packer Ave, Bethlehem, Pennsylvania 18015, United States.
² Sachdev Orthopaedics, 3735 Nazareth Rd, 302 A, Easton, Pennsylvania 18045, United States.

Abstract

The aim of this study was to analyze the proteomic differences in bone marrow aspirate concentrate (BMAC) between extraction sites, specifically, the iliac crest and humeral head. Using support vector machine (SVM) models, SVM-(linear) and SVM-(rbf), we identified six key proteins (MCSF, CD14, CD40, CD163, Ephrin-A4, and Matrilin-3) as essential for distinguishing between these sites. The results indicate that site-to-site proteomic differences are likely influenced by the surrounding tissues of each extraction site. Higher levels of chondrogenic and osteogenic proteins in the humeral head BMAC in addition to immune-modulatory proteins in the iliac crest BMAC suggest site-specific proteomic profiles. This study provides insights that may assist clinicians in selecting site-appropriate BMAC for therapeutic applications and inform future research into BMAC proteomics.