Multiple forms of cytochrome P-450 from microsomes of rat liver, kidney, and lung resolved by high-performance liquid chromatography

Biochem Int. 1985 Oct;11(4):523-31.

Abstract

The amount of cytochrome P-450 in microsomes of rat kidney and lungs was 23 and 7%, respectively, of the amount in microsomes of the liver. HPLC profiles of solubilized microsomes showed that there were five or more forms of cytochrome P-450 in microsomes of kidneys and also of lungs of untreated rats. While 3-methylcholanthrene induced a new form of cytochrome P-450 in kidney and lung microsomes, phenobarbital caused no major change in the HPLC profiles of these microsomes.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Cytochrome P-450 Enzyme System / analysis*
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Cytochrome b Group / analysis
  • Cytochrome b Group / biosynthesis
  • Cytochromes b5
  • Enzyme Induction / drug effects
  • In Vitro Techniques
  • Isoenzymes / analysis*
  • Isoenzymes / biosynthesis
  • Kidney / enzymology
  • Lung / enzymology
  • Male
  • Methylcholanthrene / pharmacology
  • Microsomes / enzymology*
  • Microsomes, Liver / enzymology
  • Mixed Function Oxygenases / biosynthesis
  • Organ Specificity
  • Phenobarbital / pharmacology
  • Rats
  • Rats, Inbred Strains

Substances

  • Cytochrome b Group
  • Isoenzymes
  • Methylcholanthrene
  • Cytochromes b5
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • Phenobarbital