Distribution of IgG subclass levels in normal adult sera as determined by a competitive enzyme immunoassay using monoclonal antibodies

Diagn Immunol. 1985;3(4):191-6.


IgG subclass levels were measured by an indirect competitive immunoenzymatic assay with selected monoclonal antibodies in sera from 180 adult blood donors. Mean levels were 6.35 mg/ml (65% of the sum of the subclasses) for IgG1, 2.61 mg/ml (26.2%) for IgG2, and 0.414 mg/ml (4.3%) for IgG3. IgG4 levels were significantly different in men (mean 0.456 mg/ml, 4.8% of the sum) and women (mean 0.29 mg/ml, 3.2%), with very wide ranges. The distribution of IgG2 levels was more homogeneous than that of IgG4 but more heterogeneous than those of IgG1 and IgG3, with a number of sera that displayed low levels of IgG2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Monoclonal*
  • Binding, Competitive
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunoglobulin G / analysis
  • Immunoglobulin G / classification*
  • Male
  • Middle Aged
  • Nephelometry and Turbidimetry
  • Statistics as Topic


  • Antibodies, Monoclonal
  • Immunoglobulin G