Exogenous prostaglandin D2 as a modulator in bovine endometritis: implications for reducing antibiotic use in dairy cattle

Xiaolin Yang; Shuangyi Zhang; Bo Liu; Lili Guo; Pengfei Gong; Jingze Wu; Yi Zhao; Wei Mao; Jinshan Cao

doi:10.3389/fvets.2025.1618203

Exogenous prostaglandin D₂ as a modulator in bovine endometritis: implications for reducing antibiotic use in dairy cattle

Front Vet Sci. 2025 Aug 12:12:1618203. doi: 10.3389/fvets.2025.1618203. eCollection 2025.

Authors

Xiaolin Yang^{1

2}, Shuangyi Zhang^{1

2}, Bo Liu^{1

2}, Lili Guo^{1

2}, Pengfei Gong^{1

2

3}, Jingze Wu^{1

2}, Yi Zhao^{1

2}, Wei Mao^{1

2}, Jinshan Cao^{1

2}

Affiliations

¹ Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Inner Mongolia Agricultural University, Hohhot, China.
² Laboratory of Veterinary Clinical Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
³ Inner Mongolia Bayannaoer City Municipal Center for Disease Control and Prevention, Linhe, China.

Abstract

Introduction: Bovine endometritis is a common postpartum uterine infection that significantly impacts the health and production performance of dairy cows, leading to economic losses for farms. Bovine endometritis is closely associated with pathogenic microorganisms, disturbances in uterine microecology, and localized inflammatory damage. Escherichia coli (E. coli) is the primary pathogenic bacterium responsible for bovine endometritis. Prostaglandin D₂ (PGD₂) is abundant in the uterine environment. However, its role in E. coli-induced endometritis remains largely unknown. We used bovine bone marrow-derived macrophages (BMDMs) and bovine endometrial tissue to investigate the specific genes and molecular mechanisms involved in E. coli-induced bovine endometritis.

Methods and results: Transcriptomic data show that E. coli infection significantly upregulated 2,141 genes and downregulated 2,381 genes in bovine BMDMs. E. coli activates various molecular functions in bovine BMDMs, with the most closely related being the inflammatory response, in which Prostaglandin-Endoperoxide Synthase 2 (PTGS2) plays a crucial role. Additionally, ELISA analysis revealed that E. coli infection significantly promoted the secretion of PGD₂ in BMDMs. In the early stage of infection, ELISA results showed that exogenous PGD₂ significantly promoted the secretion of TNF-α, IL-1β, and IL-6 in BMDMs and endometrial tissues, suggesting its role in enhancing the inflammatory response during early infection. Further q-PCR and immunofluorescence analyses demonstrated that PGD₂ markedly upregulated the expression of damage-associated molecules, including high mobility group box 1 (HMGB-1) and hyaluronic acid-binding protein 2 (HABP-2). In addition, immunofluorescence and MTT assay results indicated that PGD₂ enhanced the intracellular survival of E. coli in macrophages. H&E staining showed that PGD₂ exacerbated pathological damage in bovine endometrial tissues. Contrastingly, at later stages, PGD₂ suppresses the expression of inflammatory mediators, decreases E. coli survival, and alleviates tissue damage.

Discussion: These results not only deepen our understanding of the multifaceted role of exogenous PGD2 in uterine pathophysiology but also provide potential therapeutic implications for the treatment of bovine endometritis.

Keywords: Escherichia coli; bone marrow-derived; endometrial tissue; endometritis; prostaglandin D2.