The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions

Kristina Sturm; Michael Hothorn

doi:10.1007/978-1-0716-4799-8_12

The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions

Methods Mol Biol. 2025:2972:153-169. doi: 10.1007/978-1-0716-4799-8_12.

Authors

Kristina Sturm¹, Michael Hothorn²

Affiliations

¹ Structural Plant Biology Laboratory, Department of Plant Sciences, University of Geneva, Geneva, Switzerland.
² Structural Plant Biology Laboratory, Department of Plant Sciences, University of Geneva, Geneva, Switzerland. michael.hothorn@unige.ch.

PMID: 40879985
DOI: 10.1007/978-1-0716-4799-8_12

Abstract

Grating-coupled interferometry (GCI) is a biophysical method suitable to studying the binding kinetics of protein-ligand interactions. Here, we describe the application of biotinylated inositol pyrophosphate (PP-InsP) nutrient messengers to characterize PP-InsP-protein interactions using a Creoptix WAVEsystem.

Keywords: Binding kinetics; Creoptix WAVE; Grating-coupled interferometry; Inositol pyrophosphates; Protein-ligand interaction.

MeSH terms

Biotinylation
Inositol Phosphates* / chemistry
Inositol Phosphates* / metabolism
Interferometry* / methods
Kinetics
Ligands
Protein Binding
Proteins* / chemistry
Proteins* / metabolism

Substances

Inositol Phosphates
Ligands
Proteins