[Isolation and purification of L-lysine-alpha-oxidase from Trichoderma sp]

Vopr Med Khim. 1985 Sep-Oct;31(5):130-4.
[Article in Russian]

Abstract

An improved and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme L-lysyl-alpha-oxidase from Trichoderma sp. The method involves four steps, instead of six steps described previously, with a yield of 22.4%. The purified enzyme preparation was homogeneous as shown by polyacrylamide gel disc electrophoresis and ultracentrifugation. Physico-chemical and antitumor properties of the enzyme are under study.

Publication types

  • English Abstract

MeSH terms

  • Amino Acid Oxidoreductases / isolation & purification*
  • Chromatography, DEAE-Cellulose
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Electrophoresis, Disc
  • Mitosporic Fungi / enzymology*
  • Trichoderma / enzymology*

Substances

  • Amino Acid Oxidoreductases
  • L-lysine oxidase