A sensitive assay for active link protein from cartilage

Biochem J. 1985 Dec 1;232(2):613-6. doi: 10.1042/bj2320613.


A new assay for the activity of cartilage link protein is described. The method is based on the finding [Plaas, Sandy & Muir (1983) Biochem. J. 214, 855-864] that addition of link protein to [35S]sulphate-labelled proteoglycan aggregates from rabbit chondrocyte cultures resulted in the formation of link-stabilized aggregates. The percentage aggregate was found to be related linearly to the amount of purified bovine link protein added in the 20-120 ng range. The assay was used to monitor loss of link-protein activity during heat denaturation and to measure binding of link protein by purified proteoglycan monomer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cartilage, Articular / analysis*
  • Cells, Cultured
  • Extracellular Matrix Proteins*
  • Hot Temperature
  • Macromolecular Substances
  • Methods
  • Protein Binding
  • Protein Denaturation
  • Proteins / analysis*
  • Proteoglycans
  • Rabbits


  • Extracellular Matrix Proteins
  • Macromolecular Substances
  • Proteins
  • Proteoglycans
  • link protein