Efficient Expression and Purification of Recombinant Mouse Dimeric IgA

Eur J Immunol. 2025 Sep;55(9):e70055. doi: 10.1002/eji.70055.

Abstract

Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.

Keywords: J chain; dimeric IgA; immunoglobulin A; mucosal B cell responses; recombinant monoclonal antibody.

MeSH terms

  • Animals
  • Antibodies, Monoclonal* / genetics
  • Antibodies, Monoclonal* / immunology
  • Antibodies, Monoclonal* / isolation & purification
  • B-Lymphocytes* / immunology
  • Immunoglobulin A* / biosynthesis
  • Immunoglobulin A* / genetics
  • Immunoglobulin A* / immunology
  • Immunoglobulin A* / isolation & purification
  • Mice
  • Protein Multimerization
  • Recombinant Proteins* / genetics
  • Recombinant Proteins* / immunology
  • Recombinant Proteins* / isolation & purification

Substances

  • Immunoglobulin A
  • Recombinant Proteins
  • Antibodies, Monoclonal