Efficacy and safety of neoadjuvant treatment of trastuzumab and pyrotinib plus dalpiciclib in HR-negative/HER2-positive breast cancer: an exploratory, open-label phase II study

Int J Surg. 2026 Jan 1;112(1):865-872. doi: 10.1097/JS9.0000000000003328. Epub 2025 Sep 19.

Abstract

Background: To evaluate the efficacy and safety of a chemotherapy-free regimen consisting of monoclonal antibody trastuzumab, tyrosine kinase inhibitor pyrotinib, and CDK4/6 inhibitor dalpiciclib in patients with hormone receptor-negative/HER2-positive (HR-HER2+) early breast cancer (EBC).

Materials and methods: This open-label, single-arm, phase II study was designed using the Simon two-stage method (Registration Number: Chi-CTR-2200060748). Patients with operable HR-HER2+ EBC (T1-3 and N0-2) were enrolled. Eligible patients received trastuzumab (HLX02, 8 mg/kg loading dose, followed by 6 mg/kg every 3 weeks intravenously), pyrotinib (400 mg daily orally), and dalpiciclib (125 mg daily orally for 3 weeks, followed by 1 week off) for 16 weeks. Surgery was performed 3-6 weeks after the completion of drug treatment. The primary endpoint was total pathological complete response (tpCR, ypT0/Tis, and ypN0) rates at surgery, and secondary endpoints included breast pCR (bpCR) rates (ypT0/Tis), residual cancer burden (RCB), objective response rate (ORR), change of Ki-67 scores, survival, and safety.

Results: Between June 2022 and June 2024, a total of 34 patients with a median age of 55 years (range: 35-67) were enrolled. Thirty patients received all cycles of treatment and underwent surgery with a median follow-up of 20 months. The tpCR was achieved in 19 patients (63.3%; 95% CI, 45.5-78.1%). The bpCR was 66.7% (20/30). The number of patients with RCB-0 or RCB-I was 22 (73.3%). The most common Grade 3 treatment-related adverse events were diarrhea (50.0%), neutropenia (20.6%), and leukopenia (17.7%). No Grade 4 events or treatment-related deaths occurred.

Conclusion: In patients with HR-HER2+ EBC, the neoadjuvant therapy with trastuzumab, pyrotinib, and dalpiciclib has promising activity and manageable toxicity. Further investigation is needed.

Keywords: HR-negative/HER2-positive breast cancer; chemotherapy-free; dalpiciclib; neoadjuvant therapy.

Publication types

  • Clinical Trial, Phase II

MeSH terms

  • Acrylamides* / administration & dosage
  • Acrylamides* / adverse effects
  • Acrylamides* / therapeutic use
  • Adult
  • Aged
  • Aminoquinolines* / administration & dosage
  • Aminoquinolines* / adverse effects
  • Aminoquinolines* / therapeutic use
  • Antineoplastic Combined Chemotherapy Protocols* / adverse effects
  • Antineoplastic Combined Chemotherapy Protocols* / therapeutic use
  • Breast Neoplasms* / drug therapy
  • Breast Neoplasms* / metabolism
  • Breast Neoplasms* / pathology
  • Breast Neoplasms* / surgery
  • Erb-b2 Receptor Tyrosine Kinases / metabolism
  • Female
  • Humans
  • Middle Aged
  • Neoadjuvant Therapy* / methods
  • Piperidines
  • Pyridines
  • Pyrimidines
  • Trastuzumab* / administration & dosage
  • Trastuzumab* / adverse effects
  • Trastuzumab* / therapeutic use
  • Treatment Outcome

Substances

  • Trastuzumab
  • Acrylamides
  • pyrotinib
  • Erb-b2 Receptor Tyrosine Kinases
  • Aminoquinolines
  • ERBB2 protein, human
  • dalpiciclib
  • Piperidines
  • Pyridines
  • Pyrimidines