Diagnostic and Prognostic Performance of the Agile 3+ Score in Patients With Chronic Hepatitis B and Concurrent MASLD After Complete Viral Suppression With Nucleos(t)ide Analogues

J Gastroenterol Hepatol. 2025 Dec;40(12):2963-2975. doi: 10.1111/jgh.70099. Epub 2025 Oct 1.

Abstract

Background: The applicability of the Agile 3+ score to patients with CHB-MASLD receiving antiviral therapy remains unexplored. Our objective was to explore the diagnostic and prognostic performances of the Agile 3+ score in patients with CHB-MASLD.

Methods: This retrospective cohort study included consecutively enrolled patients with CHB and concurrent MASLD from 2014 to 2020. In Part 1, we evaluated the performance of the Agile 3+ score in predicting advanced fibrosis. In Part 2, the Agile 3+ score was applied to a follow-up cohort to assess its correlation with liver-related events.

Results: Among the 2492 patients evaluated by vibration-controlled transient elastography, 103 patients with CHB and hepatic steatosis were included in the biopsy cohort, and 716 patients were included in the follow-up cohort. The Agile 3+ score had the highest AUROC of 0.916 for the prediction of advanced fibrosis, outperforming the FIB-4 score (0.845), NFS (0.838), and APRI (0.726). In predicting liver-related events (LREs), the AUROC of the Agile 3+ score was also significantly higher (0.795 at 3 years, 0.809 at 5 years, and 0.834 at 8 years) than those of the other noninvasive tests. When the recommended cutoffs (0.451 and 0.679) were applied for MASLD risk stratification, the high-risk group (HR = 13.61) and intermediate-risk group (HR = 3.53) had significantly greater risks of LREs than the low-risk group.

Conclusion: In patients with CHB and concurrent MASLD receiving antiviral therapy, the Agile 3+ score effectively identifies advanced fibrosis and predicts LRE risk, supporting its clinical utility in this population.

Keywords: chronic hepatitis B; hepatic steatosis; liver fibrosis; noninvasive test.

MeSH terms

  • Adult
  • Antiviral Agents* / therapeutic use
  • Elasticity Imaging Techniques
  • Female
  • Follow-Up Studies
  • Hepatitis B, Chronic* / complications
  • Hepatitis B, Chronic* / diagnosis
  • Hepatitis B, Chronic* / drug therapy
  • Hepatitis B, Chronic* / virology
  • Humans
  • Liver Cirrhosis* / diagnosis
  • Liver Cirrhosis* / etiology
  • Male
  • Middle Aged
  • Nucleosides* / therapeutic use
  • Predictive Value of Tests
  • Prognosis
  • Retrospective Studies
  • Severity of Illness Index
  • Sustained Virologic Response

Substances

  • Antiviral Agents
  • Nucleosides