Prevalence, Species Diversity, and Risk Factors of Tick Infestation in Cattle From District Peshawar, Pakistan

Abstract

This study investigated the prevalence, diversity, and risk factors of tick infestation and Theileria annulata infection in cattle from District Peshawar, Pakistan. A total of 322 cattle of different breeds, ages, and sexes were examined between January and March 2024. The overall prevalence of tick infestation was 35.4%, with females more frequently infested than males. Exotic and crossbred cattle, particularly Holstein Friesian and Jersey, along with calves, showed higher susceptibility compared to indigenous breeds and older cattle. Morphological identification revealed Rhipicephalus microplus as the most prevalent species, followed by Hyalomma anatolicum anatolicum, while other species occurred at lower frequencies. Species distribution varied significantly across breeds, with R. microplus predominating in Sahiwal cattle and H. anatolicum strongly associated with Holstein Friesian. Molecular screening confirmed T. annulata infection, with the highest prevalence detected in female H. anatolicum, whereas R. microplus showed relatively low infection rates. Ecological analysis indicated moderate species diversity overall, with Holstein Friesian cattle exhibiting the greatest richness and dissimilarity in tick fauna compared to indigenous breeds. Tick burden severity was mostly moderate, and co-infestation patterns were dominated by combinations of H. anatolicum and R. microplus. This study is the first to integrate molecular detection of T. annulata with ecological indices of tick diversity in cattle of Peshawar, providing a comprehensive picture of vector-host-pathogen interactions. The identification of breed- and age-specific vulnerabilities highlights the need for targeted tick control strategies. These findings have direct implications for designing sustainable management programs aimed at reducing the economic and health burden of tropical theileriosis in endemic regions.

Keywords: Theileria annulata; cattle breeds; epidemiological analysis; tick infestation; vector‐borne diseases.