Antigenic relationships in mammalian DNA polymerase

Science. 1972 Mar 10;175(4026):1116-7. doi: 10.1126/science.175.4026.1116.


Rabbit antibody was prepared against a high-molecular-weight DNA polymerase purified from the soluble fraction of calf thymus gland. This antibody does not inhibit terminal deoxynucleotidyl transferase isolated from that source, but does inhibit both low-molecular-weight and high-molecular-weight DNA polymerases isolated from cytoplasmic and nuclear fractions of a number of mammalian tissues (mouse L cells, calf thymus, phytohemagglutinin-stimulated human lymphocytes, rat liver, and rabbit bone marrow). The results suggest that (i) no antigenic relationship exists between terminal transferase and DNA polymerase, (ii) common antigenic determinants exist in the DNA polymerases from all mammalian sources, and (iii) multiple forms of DNA polymerase found in mammalian, cells are related by having polypeptide sequences or subunits in common.

MeSH terms

  • Animals
  • Antigen-Antibody Reactions*
  • Bone Marrow Cells
  • Cattle
  • Cell Nucleus / enzymology
  • Cells, Cultured / immunology
  • Cross Reactions
  • Cytoplasm / enzymology
  • DNA Nucleotidyltransferases / analysis*
  • Epitopes*
  • Escherichia coli / immunology
  • Humans
  • Immune Sera
  • L Cells
  • Lectins / pharmacology
  • Liver
  • Liver Regeneration
  • Lymphocytes / drug effects
  • Mice
  • Mitochondria / enzymology
  • Molecular Weight
  • Rabbits
  • Rats
  • Thymus Gland


  • Epitopes
  • Immune Sera
  • Lectins
  • DNA Nucleotidyltransferases