The synthetic estrogen 17α-ethinylestradiol (EE2), commonly found in aquatic environments, poses significant ecological threats. This study explores the enzymatic potential of 18 macrofungal species, primarily indigenous mushroom strains from Thailand, to produce laccase and degrade EE2. These native species may possess unique enzymatic properties that make them well suited for environmental applications. The novelty of this research lies in the comparative evaluation of these diverse mushroom species. Laccase activity was assessed using both qualitative (ABTS-PDA colorimetric assay) and quantitative (U/L) methods, while EE2 degradation was analyzed via high-performance liquid chromatography (HPLC). Most strains exhibited laccase activity by day 7 at 28°C, with six strains showing strong qualitative responses. Notably, Coprinus comatus No. 1 demonstrated the highest activity (109,685 U/L) on day 11. All strains exhibited the ability to degrade EE2, with six strains achieving 100% removal efficiency after only 3 days of enzymatic treatment. A strong positive correlation (r = 0.79) was observed between laccase activity and EE2 degradation, supporting the potential of fungal enzymes in pollutant remediation. These findings highlight several promising fungal candidates for eco-friendly and sustainable degradation technologies.
Keywords: 17 α‐ethinylestradiol removal; enzymatic degradation; fungal screening; laccase production.
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