Purpose: To compare oxygen saturation measurements when pulse oximeter sensors were simultaneously placed on the index finger and the second toe, and compare blood pressure recordings with non-invasive blood pressure (NIBP) cuffs simultaneously placed on the upper arm and the lower calf in children receiving general anesthesia. Methods: A total of 50 children, aged three to nine years, defined as ASA Class I or II receiving comprehensive dental treatment in the operating room with general anesthesia, were included. Pulse oximeter sensors were simultaneously placed on each subject's index finger and ipsilateral second toe. Non-invasive blood pressure cuffs were simultaneously placed on the upper arm and ipsilateral lower calf above the ankle. The same anesthesia regimen was used on all patients. Following induction of general anesthesia, measurements were simultaneously recorded every five minutes during the dental procedure. Results: Index finger oxygen saturation measurements were significantly lower than the second toe (P<0.001). Arm systolic blood pressure measurements were significantly lower than calf measurements (P<0.001). Arm diastolic blood pressure measurements were significantly lower than calf measurements (P<0.001). Conclusions: Discrepancy of vital sign measurements may be notable depending on the site from which measurements are obtained. If blood pressure is taken on the calf of a child in the Trendelenburg position, the reading will likely produce a higher systolic and diastolic measurement and should be interpreted with caution. A pulse oximeter placed on the first finger or second toe of healthy children can be regarded as interchangeable in a clinical setting.