Background: In early atherosclerosis, circulating Low-Density Lipoprotein (LDL) crosses the endothelium by transcytosis. This involves caveolar uptake of LDL by scavenger receptor BI (SR-BI) and activin-like kinase 1 (ALK1) and requires the protein caveolin-1 (Cav-1). We identified mediators of LDL transcytosis by isolating membrane microdomains enriched in caveolin-1 from human coronary endothelial cells (HCAECs) treated with LDL and performing mass spectrometry. One of the proteins identified was myosin-9 (MYH9).
Methods: Total internal reflection fluorescence microscopy was conducted to measure LDL transcytosis by HCAECs. We measured LDL transcytosis in vivo in mice lacking endothelial MYH9 (EC-Myh9-/-). Atherosclerosis studies were also performed in EC-Myh9-/- deleted of hepatic LDLR via (adeno-associated virus, AAV)-CRISPR. Additionally, we performed analysis of human transcriptomic data.
Results: Gene ontology analysis in human aortic endothelial cells suggested a role for MYH9 in exocytosis. Both knockdown and pharmacologic inhibition of MYH9 inhibited LDL transcytosis. MYH9 depletion caused an accumulation of LDL-containing vesicles at the base of the cell; overexpression caused an increase in LDL exocytosis. EC-Myh9-/- mice accumulated less LDL in the aortic arch after acute injection with LDL. To investigate the role of MYH9 in atherosclerosis, we deleted hepatic LDL in EC-Myh9-/- mice using AAV-CRISPR and fed them a high-fat diet. The aortic arch and root of AAV-CRISPR; EC-Myh9-/- mice exhibited smaller plaques. Human transcriptomic data showed greater messenger RNA (mRNA) levels of aortic MYH9 in atherosclerotic aortas compared to healthy controls.
Conclusions: Lipid raft proteomics identified MYH9 as a regulator of LDL transcytosis. MYH9 is required for endothelial LDL exocytosis and contributes to early atherosclerosis.
Keywords: LDL; MYH9; atherosclerosis; endothelium; transcytosis.