Urokinase plasminogen activator receptor attenuates allergen-induced eosinophil migration and airway hyperresponsiveness

Abstract

Rationale: The urokinase plasminogen activator receptor (uPAR) is a membrane-bound protein that can contribute to the activation and mobilization of leukocytes and is present at increased levels in asthmatics. However, its role in allergic asthma remains poorly understood.

Methods: We used multiple mouse strains and different models of allergic airway disease to study the function of uPAR in the pathogenesis of this disease.

Measurements and main results: Plaur, the gene encoding uPAR, was rapidly induced following allergic sensitization through the airway and again following subsequent allergen challenge. Plaur-deficient mice displayed both increased numbers of eosinophils and heightened airway hyperresponsiveness (AHR) in multiple models of allergic asthma. Mice selectively lacking Plaur in eosinophils also had more robust eosinophilia than did wild-type (WT) mice, and eosinophils lacking Plaur displayed increased activity in an ex vivo assay of chemokine-dependent migration. However, those mice did not have increased AHR compared with WT mice. Conversely, although mice selectively lacking Plaur in lung epithelial cells did not have increased inflammation compared with WT mice, they displayed heightened AHR.

Conclusions: These findings suggest that uPAR controls both airway inflammation and AHR, but through distinct mechanisms. Targeting uPAR might have therapeutic potential for treating inflammation and AHR in asthma.

Keywords: Plaur; asthma; eosinophils; mice; uPAR.