Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 69 (7), 1934-8

Demonstration That Antigen-Binding Cells Are Precursors of Antibody-Producing Cells After Purification With a Fluorescence-Activated Cell Sorter

Demonstration That Antigen-Binding Cells Are Precursors of Antibody-Producing Cells After Purification With a Fluorescence-Activated Cell Sorter

M H Julius et al. Proc Natl Acad Sci U S A.

Abstract

We have obtained viable and functional populations of antigen-binding cells enriched up to 500-fold from primed spleen-cell suspensions by fluorescent labeling and by a new electronic cell sorter that sorts viable cells according to fluorescence. Concomitantly, populations largely depleted of antigen-binding cells were obtained. While neither population alone is capable of a full adoptive secondary response when injected into irradiated recipients, a reconstituted mixture restores the full response of the unfractionated spleen cells. Admixture of sources of unprimed thymus-derived cells (T-cells) with the purified antigen-binding cells (B-cells) restores much of the full response.

Similar articles

See all similar articles

Cited by 51 articles

See all "Cited by" articles

References

    1. Int Arch Allergy Appl Immunol. 1966;30(5):446-55 - PubMed
    1. Transplantation. 1967 Nov;5(6):1484-95 - PubMed
    1. Immunochemistry. 1969 Jan;6(1):67-76 - PubMed
    1. Nature. 1969 Jun 28;222(5200):1291-2 - PubMed
    1. Immunology. 1969 Oct;17(4):503-16 - PubMed

LinkOut - more resources

Feedback