The effect of banol and paraoxon on the NADPH-cytochrome c reductase activity and cytochrome P-450 content in rats

Bull Environ Contam Toxicol. 1977 Oct;18(4):472-7. doi: 10.1007/BF01683719.

Abstract

We investigated the effect of banol and paraoxon on the activity of NADPH-cytochrome c reductase and cytochrome P-450 concentration in microsomes of rats treated in vivo with these compounds alone, or in the presence of phenobarbitone. The results showed that banol reduced the NADPH-cytochrome c reductase activity and the concentration of cytochrome P-450, while paraoxon reduced the activity of NADPH-cytochrome c reductase, but increased the cytochrome P-450 concentration. Phenobarbitone stimulated an increased NADPH-cytochrome c reductase activity and cytochrome P-450 concentration. When applied with banol and paraoxon, phenobarbitone lowered their inhibitory effect.

MeSH terms

  • Animals
  • Carbamates*
  • Cytochrome P-450 Enzyme System / metabolism*
  • Cytochrome Reductases / metabolism*
  • Drug Interactions
  • Female
  • In Vitro Techniques
  • Insecticides / pharmacology*
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / enzymology*
  • NADPH-Ferrihemoprotein Reductase / metabolism*
  • Paraoxon / pharmacology*
  • Phenobarbital / pharmacology
  • Proteins / metabolism
  • Rats
  • Xylenes / pharmacology

Substances

  • Carbamates
  • Insecticides
  • Proteins
  • Xylenes
  • Cytochrome P-450 Enzyme System
  • Cytochrome Reductases
  • NADPH-Ferrihemoprotein Reductase
  • carbanolate
  • Paraoxon
  • Phenobarbital