Dark septate endophytes promote the growth of Cynodon dactylon under drought stress and enhance its potential for use in the ecological restoration of slopes

Haoji Jia; Qiming Geng; Mingyi Li; Ran Wang; Fuhao Wang; Yuxin Deng; Wennian Xu; Daxiang Liu

doi:10.3389/fpls.2025.1537256

Dark septate endophytes promote the growth of Cynodon dactylon under drought stress and enhance its potential for use in the ecological restoration of slopes

Front Plant Sci. 2025 Oct 21:16:1537256. doi: 10.3389/fpls.2025.1537256. eCollection 2025.

Authors

Haoji Jia^{1

2}, Qiming Geng¹, Mingyi Li^{1

2}, Ran Wang^{1

2}, Fuhao Wang^{1

2}, Yuxin Deng^{2

3}, Wennian Xu^{1

2}, Daxiang Liu^{1

2}

Affiliations

¹ Key Laboratory of Geological Hazards on Three Gorges Reservoir Area (China Three Gorges University), Ministry of Education, Yichang, China.
² Hubei Provincial Engineering Research Center of Slope Habitat Construction Technique Using Cement-based Material (China Three Gorges University), Yichang, China.
³ College of Biological & Pharmaceutical Sciences, China Three Gorges University, Yichang, China.

Abstract

Introduction: The objective of this study was to investigate whether dark septate endophytes (DSEs) can increase plant drought tolerance in the context of vegetation concrete, which is a complex environment.

Methods: This study employed a controlled simulation experiment to investigate the influence of inoculation with diverse DSEs, namely, Paraphoma chrysanthemicola (PC), Alternaria alternata (AA), and Cladosporium cladosporioides (CC), on the growth, photosynthetic characteristics, osmoregulatory substance content, and antioxidant enzyme activities of Cynodon dactylon in vegetation concrete subjected to drought stress.

Results: These findings demonstrated that DSEs were capable of effectively mitigating the adverse impacts of drought on plant growth. Under moderate drought (MD 55%±5% of the maximum moisture capacity in the field), DSEs increased the dry weight (DB), net photosynthetic rate (Pn), soluble sugar (SS) and peroxidase (POD) of C. dactylon by up to 14.21%, 32.63%, 40.73% and 31.43%, respectively, and reduced the malondialdehyde (MDA) content by 8.02-13.77%. Furthermore, under severe drought (SD, 35%±5% of the maximum moisture capacity in the field), DSE inoculation enhanced the photosynthetic capacity of C. dactylon, stimulated the accumulation of osmoregulatory compounds such as proline (Pro) and soluble protein (SP), and mitigated the water loss associated with drought.

Conclusion: The results demonstrate that DSE inoculation enhances the drought resistance of plants used in vegetation concrete by increasing the photosynthetic rate, and contents of antioxidant enzymes and osmoregulatory substances. This study provides reference for the use of DSEs in ecological restoration with vegetation concrete.

Keywords: Cynodon dactylon; antioxidant enzyme activities; dark septate endophytes; drought; osmoregulation substance; vegetation concrete.