A genotype-independent and highly efficient Agrobacterium-mediated soybean genetic transformation system

Fengxue Jing; Leping Geng; Yanyan Zhang; Hongtao Xie; Jian-Kang Zhu; Jianhua Zhu

doi:10.1111/jipb.70102

A genotype-independent and highly efficient Agrobacterium-mediated soybean genetic transformation system

J Integr Plant Biol. 2025 Dec 3. doi: 10.1111/jipb.70102. Online ahead of print.

Authors

Fengxue Jing^{1

2}, Leping Geng^{1

3}, Yanyan Zhang^{1

3}, Hongtao Xie^{1

2}, Jian-Kang Zhu⁴, Jianhua Zhu^{1

3}

Affiliations

¹ School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
² Bellagen Biotechnology Co., Ltd, Jinan, 250000, China.
³ Research Center for Biological Breeding, Advanced Academy, Anhui Agricultural University, Hefei, 230036, China.
⁴ Southern University of Science and Technology, Shenzhen, 518055, China.

PMID: 41334854
DOI: 10.1111/jipb.70102

Abstract

A stable and efficient transformation system is crucial for functional genomics and trait improvement in soybean. This study developed a tissue culture based genetic transformation system incorporating dual selection (Spectinomycin and RUBY). This system significantly enhances transformation efficiency, shortens the transformation cycle, and demonstrates broad genotype independence, providing a powerful tool for soybean research and breeding.

Keywords: Agrobacterium; genetic transformation; genotype‐independent; soybean.