IL-27 promotes Treg cell expression of CD122 and fitness at homeostasis

Proc Natl Acad Sci U S A. 2025 Dec 16;122(50):e2519141122. doi: 10.1073/pnas.2519141122. Epub 2025 Dec 9.

Abstract

Regulatory T (Treg) cells express high levels of the IL-27R, and in the setting of infection and autoimmunity, the cytokine IL-27 promotes Treg cell activities that mitigate tissue pathology. However, IL-27 appears dispensable for Treg cell development and maintenance as lineage-specific depletion of the IL-27R on Treg cells does not impact these populations at steady state. In contrast, when mice were generated in which the Treg compartment comprised a mix of IL-27R-sufficient and -deficient Treg cells, those that lacked IL-27R were at a competitive disadvantage. Aging experiments illustrate that IL-27R-deficient Treg cells are preferentially eroded, and this defect was associated with reduced expression of CD122, the β chain of the IL-2/15R. Moreover, blockade of CD122 led to a similar loss of Treg cells, and in vitro and in vivo studies highlight that IL-27 promotes Treg cell expression of CD122 and improves responsiveness to IL-2/15. These datasets reveal that homeostatic IL-27 signals provide a competitive advantage that shapes the composition of the Treg cell pool by modulating responsiveness to growth factors.

Keywords: IL-27; Treg; competition; cytokine; homeostasis.

MeSH terms

  • Animals
  • Homeostasis* / immunology
  • Interleukin-2
  • Interleukin-2 Receptor beta Subunit* / genetics
  • Interleukin-2 Receptor beta Subunit* / immunology
  • Interleukin-2 Receptor beta Subunit* / metabolism
  • Interleukin-27* / metabolism
  • Interleukins* / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • T-Lymphocytes, Regulatory* / immunology
  • T-Lymphocytes, Regulatory* / metabolism

Substances

  • Interleukin-2 Receptor beta Subunit
  • Interleukin-27
  • Interleukins
  • Il27 protein, mouse
  • Interleukin-2
  • Il2rb protein, mouse