A dataset on the phylogeny of Mycobacterium avium subsp. paratuberculosis isolated from patients in a referral gastrointestinal diseases centre in the Sudan

Sanaa M Idris; Wisal A Elmagzoub; Maha Isameldin; Nassir Arabi; Abdelmonem Abdo; Mustafa Ibrahim; Sahar M Bakhiet; Julius B Okuni; Lonzy Ojok; Ahmed A Gameel; Uwe Truyen; Ahmad Amanzada; Ahmed Abd El Wahed; Kamal H Eltom; ElSagad Eltayeb

doi:10.1016/j.dib.2025.112257

A dataset on the phylogeny of Mycobacterium avium subsp. paratuberculosis isolated from patients in a referral gastrointestinal diseases centre in the Sudan

Data Brief. 2025 Nov 11:63:112257. doi: 10.1016/j.dib.2025.112257. eCollection 2025 Dec.

Authors

Sanaa M Idris^{1

2

3}, Wisal A Elmagzoub^{1

2

4}, Maha Isameldin⁵, Nassir Arabi^{5

6}, Abdelmonem Abdo⁷, Mustafa Ibrahim⁵, Sahar M Bakhiet⁸, Julius B Okuni⁹, Lonzy Ojok^{9

10}, Ahmed A Gameel³, Uwe Truyen¹, Ahmad Amanzada¹¹, Ahmed Abd El Wahed¹, Kamal H Eltom^{1

2}, ElSagad Eltayeb^{5

12}

Affiliations

¹ Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.
² Department of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Shambat, Khartoum North, Sudan.
³ Department of Pathology, Faculty of Veterinary Medicine, University of Khartoum, Shambat, Khartoum North, Sudan.
⁴ Department of Biology and Biotechnology, College of Applied and Industrial Sciences, University of Bahri, Khartoum North, Sudan.
⁵ Ibn Sina Specialised Hospital, Khartoum, Sudan.
⁶ Faculty of Medicine and Health Sciences, Omdurman Islamic University, Omdurman, Sudan.
⁷ National Centre for Gastroenterology and Liver Diseases, Ministry of Health, Khartoum, Sudan.
⁸ Department of Molecular Biology, Institute of Endemic diseases, University of Khartoum, Sudan.
⁹ College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, Kampala, Uganda.
¹⁰ Department of Pathology, Faculty of Medicine, Gulu University, Gulu, Uganda.
¹¹ Department of Gastroenterology and Gastrointestinal Oncology, University Medical Centre Göttingen, Göttingen, Germany.
¹² Faculty of Medicine, Al Neelain University, Khartoum, Sudan.

Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis in animals, a disease characterized by chronic intestinal inflammation. MAP has also been detected in patients with Crohn's disease and other illnesses, raising concerns about its potential public health implications. Recently, we isolated MAP from tissue and stool samples of human patients with gastrointestinal complaints for the first time in the Sudan. This dataset aimed at investigating the phylogenetic relationships of these Sudanese MAP human isolates to local animal MAP isolates and MAP strains worldwide. DNA was extracted from MAP cultures of patient tissues and stools, partially amplified for the MAP IS1311 gene using nested PCR, and then sequenced. High-quality sequences of 13 isolates (2 stool and 11 tissue isolates) together with corresponding sequences of Sudanese MAP animal isolates and of MAP strains from other countries were used to construct a phylogenetic tree. The phylogeny tree showed that the Sudanese human MAP isolates are closely related to the Sudanese animal isolates and to the MAP S-type (Sheep-type) strains from other countries including Australia, the USA, Germany, and New Zealand.

Keywords: Gastrointestinal complaints; Human isolates; Mycobacterium avium subsp. paratuberculosis; Paratuberculosis; Sudan.