The bacterial degradation of flavonoids. Hydroxylation of the A-ring of taxifolin by a soil pseudomonad

Biochem J. 1972 Nov;130(2):373-81. doi: 10.1042/bj1300373.

Abstract

Cell-free extracts prepared from a Pseudomonas sp. grown on (+)-catechin oxidized taxifolin in the presence of NAD(P)H and molecular oxygen. The enzyme catalysing the reaction was partially purified and shown to be a flavoprotein. The product was identified as 3',4',5,7,8-pentahydroxyflavanonol (dihydrogossypetin).

MeSH terms

  • Catechols / metabolism
  • Cell-Free System
  • Chromatography, Thin Layer
  • Culture Media
  • Electrophoresis, Disc
  • Flavonoids / metabolism*
  • Flavoproteins / analysis
  • Hydroxylation
  • Mixed Function Oxygenases / analysis
  • NADP
  • Oxidation-Reduction
  • Oxygen
  • Oxygenases / analysis
  • Pseudomonas / enzymology
  • Pseudomonas / metabolism*
  • Soil Microbiology
  • Spectrophotometry

Substances

  • Catechols
  • Culture Media
  • Flavonoids
  • Flavoproteins
  • NADP
  • Mixed Function Oxygenases
  • Oxygenases
  • Oxygen