Abstract
Cell-free extracts prepared from a Pseudomonas sp. grown on (+)-catechin oxidized taxifolin in the presence of NAD(P)H and molecular oxygen. The enzyme catalysing the reaction was partially purified and shown to be a flavoprotein. The product was identified as 3',4',5,7,8-pentahydroxyflavanonol (dihydrogossypetin).
MeSH terms
-
Catechols / metabolism
-
Cell-Free System
-
Chromatography, Thin Layer
-
Culture Media
-
Electrophoresis, Disc
-
Flavonoids / metabolism*
-
Flavoproteins / analysis
-
Hydroxylation
-
Mixed Function Oxygenases / analysis
-
NADP
-
Oxidation-Reduction
-
Oxygen
-
Oxygenases / analysis
-
Pseudomonas / enzymology
-
Pseudomonas / metabolism*
-
Soil Microbiology
-
Spectrophotometry
Substances
-
Catechols
-
Culture Media
-
Flavonoids
-
Flavoproteins
-
NADP
-
Mixed Function Oxygenases
-
Oxygenases
-
Oxygen