A SWI/SNF-specific Ig-like domain, SWIFT, is a transcription factor binding platform

Science. 2026 Apr 2;392(6793):eaeb3627. doi: 10.1126/science.aeb3627. Epub 2026 Apr 2.

Abstract

Mammalian switch/sucrose nonfermenting (mSWI/SNF) chromatin remodeling complexes modulate DNA accessibility and gene expression; however, their genomic targeting mechanisms remain incompletely understood. Here, we identify SWIFT [SWI/SNF immunoglobulin fold (Ig-fold) for transcription factor interactions], a conserved transcription factor (TF) binding domain on the SMARCD subunits. SWIFT is necessary and sufficient for direct engagement with the transactivation domain of the PU.1 TF. A single amino acid mutation disrupts PU.1-mSWI/SNF binding, impairs complex targeting, and attenuates oncogenic transcription and proliferation in PU.1-dependent human cancer cells. Dominant expression of the SWIFT domain in isolation sequesters TFs from mSWI/SNF and poisons TF-"addicted" cancer cells. Finally, TFs across diverse families interact with SMARCD paralog-specific SWIFT domains. These results define a major mechanism of cell type- and disease-specific mSWI/SNF chromatin targeting and inform approaches toward therapeutic modulation.

MeSH terms

  • Cell Proliferation
  • Chromatin / metabolism
  • Chromatin Assembly and Disassembly
  • Chromosomal Proteins, Non-Histone* / chemistry
  • Chromosomal Proteins, Non-Histone* / genetics
  • Chromosomal Proteins, Non-Histone* / metabolism
  • HEK293 Cells
  • Humans
  • Mutation
  • Protein Binding
  • Protein Domains
  • Proto-Oncogene Proteins* / chemistry
  • Proto-Oncogene Proteins* / genetics
  • Proto-Oncogene Proteins* / metabolism
  • Trans-Activators* / chemistry
  • Trans-Activators* / genetics
  • Trans-Activators* / metabolism
  • Transcription Factors* / chemistry
  • Transcription Factors* / genetics
  • Transcription Factors* / metabolism

Substances

  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Proto-Oncogene Proteins
  • SWI-SNF-B chromatin-remodeling complex
  • Trans-Activators
  • Transcription Factors
  • SMARCD1 protein, human
  • SMARCD2 protein, human
  • SMARCD3 protein, human