Prognostic value of T-lymphocyte subsets, lymphocyte-to-high-density lipoprotein ratio, interleukin 6, C-reactive protein and procalcitonin in patients with septic shock and effects of vitamin C on patients with septic shock

Pak J Pharm Sci. 2026 Jan;39(1):164-173. doi: 10.36721/PJPS.2026.39.1.REG.14162.1.

Abstract

Background: Septic shock remains a critical condition with high mortality, necessitating reliable prognostic biomarkers and effective adjunct therapies.

Objectives: This study explored prognostic biomarkers and the effect of vitamin C in septic shock.

Methods: This study analyzed 110 patients (January 2023-March 2024), stratified by 28-day outcome into survival (n=90) and death (n=20) groups. Compared to survivors, the death group exhibited significantly lower lymphocyte-to-high-density lipoprotein ratio (LHR), CD3+, CD3+CD4+, CD4+/CD8+ and higher interleukin 6 (IL-6), C-reactive protein (CRP), procalcitonin (PCT), CD3+CD8+.

Results: The receiver operating characteristic analysis showed the combination of LHR, IL-6, CRP, PCT and CD4+/CD8+ predicted death best [area under the receiver operating characteristic curve (AUC) =0.960], outperforming single markers. Patients were randomized to control (hydrocortisone) or observation (hydrocortisone with vitamin C) group. Post-treatment, both groups showed improved mean arterial pressure (MAP), central venous pressure (CVP) (increased), heart rate (HR) (decreased) and reduced PCT, tumor necrosis factor-α (TNF-α), IL-6 and Sequential Organ Failure Assessment (SOFA) score; however, improvements were significantly greater in the vitamin C group.

Conclusion: The combination of LHR, IL-6, CRP, PCT and CD4+/CD8+ has prognostic value. Vitamin C adjunct therapy significantly enhances hemodynamic improvement, reduces inflammation, lowers SOFA scores and improves prognosis in septic shock patients.

Keywords: C-reactive protein; Interleukin 6; Lymphocyte-to-high-density lipoprotein ratio; Procalcitonin; Prognosis; Septic shock; T-lymphocyte subsets; Vitamin C.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Aged
  • Ascorbic Acid* / therapeutic use
  • Biomarkers / blood
  • C-Reactive Protein* / metabolism
  • Female
  • Humans
  • Interleukin-6* / blood
  • Lipoproteins, HDL* / blood
  • Male
  • Middle Aged
  • Procalcitonin* / blood
  • Prognosis
  • Shock, Septic* / blood
  • Shock, Septic* / diagnosis
  • Shock, Septic* / drug therapy
  • Shock, Septic* / immunology
  • Shock, Septic* / mortality
  • T-Lymphocyte Subsets* / drug effects
  • T-Lymphocyte Subsets* / immunology

Substances

  • Procalcitonin
  • C-Reactive Protein
  • Ascorbic Acid
  • Interleukin-6
  • Biomarkers
  • Lipoproteins, HDL
  • IL6 protein, human