The seminal plasma proteome dataset of the giant panda

Shili Zhou; Tao Wang; Jiasong Chen; Feiping Li; Shenfei Wang; Xianbiao Hu; Mingyue Zhang; Rong Hou; Yuliang Liu; Kailai Cai

doi:10.1016/j.dib.2025.112419

The seminal plasma proteome dataset of the giant panda

Data Brief. 2025 Dec 24:64:112419. doi: 10.1016/j.dib.2025.112419. eCollection 2026 Feb.

Authors

Shili Zhou¹, Tao Wang², Jiasong Chen^{3

4}, Feiping Li^{3

4}, Shenfei Wang^{3

4}, Xianbiao Hu^{3

4}, Mingyue Zhang^{3

4}, Rong Hou^{3

4}, Yuliang Liu^{3

4}, Kailai Cai^{3

4}

Affiliations

¹ School of Pharmacy, Chengdu University, Chengdu 610106, China.
² School of Basic Medical Sciences, Chengdu University, Chengdu 610106, China.
³ Chengdu Research Base of Giant Panda Breeding, Chengdu 610081 Sichuan, China.
⁴ The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu 610081 Sichuan, China.

Abstract

For the ex-situ conservation of giant pandas, both collecting and preserving semen are important methods. The seminal plasma is rich in nutrients and bioactive substances, such as proteins, carbohydrates, lipids, amino acids, and hormones, which play an important role in the reproduction and reproductive health of the species. Understanding the composition and function of proteins in the giant panda seminal plasma proteome can provide valuable insights into their reproductive biology and help develop strategies to improve their reproductive success in captivity, which is essential for giant panda conservation. This is the first study to analyze the seminal plasma proteins of giant pandas through proteomics, identifying 1125 proteins. These proteins are related to protein turnover, translation, and metabolism. The seminal plasma proteins of giant pandas were then compared to those of humans, pigs, and sheep, and many unique proteins were found in giant panda samples. Among these proteins, the WD40 repeat-containing proteins have been identified and implicated in sperm function and fertility.

Keywords: Giant panda; Proteome; Seminal plasma; WD40 repeats proteins.